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常规 rt-pcr 和 sybr 绿色 rt-qpcr 检测罗非鱼
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Medio ambiente
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JoVE Revista Medio ambiente
Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR
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常规 rt-pcr 和 sybr 绿色 rt-qpcr 检测罗非鱼

25,981 Views

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10:55 min

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November 10, 2018

DOI:

10.3791/58596-v

DOI:
10.3791/58596-v

•

10:55 min
November 10, 2018

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11 Views
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1Institute of Veterinary Bacteriology Vetsuisse Faculty,University of Bern, 2Department of Veterinary Microbiology and Immunology and Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies,Kasetsart University

Tags

Tilapia Lake VirusRT-PCRSYBR Green RT-qPCRSample CollectionRNA ExtractionReal-time PCRVirus DetectionTilapiaAquacultureEmerging Viral DiseaseFood Security

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Nicholson, P., Rawiwan, P., Surachetpong, W. Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR. J. Vis. Exp. (141), e58596, doi:10.3791/58596 (2018).

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