Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
1Lab of Animal Models and Functional Genomics (LAMFG), The Key Laboratory of Animal Vaccine & Protein Engineering, College of Veterinary Medicine,Hunan Agricultural University (HUNAU), 2Department of Pathology,Georgetown University Medical School, 3College of Food Science and Technology,Hunan Agricultural University (HUNAU), 4Lab of Molecular Cardiology (LMC),National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH), 5Transgenic Core,National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH)
Capítulos
- 00:04Título
- 01:02Southern Blotting Screening
- 04:40Results: Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells
- 06:07Conclusion
Vi præsenterer her, en detaljeret protokol for at identificere homologe rekombination begivenheder, der fandt sted i mus embryonale stamceller ved hjælp af det sydlige blotting og/eller PCR. Denne metode er eksemplificeret ved generation af nonmuscle myosin II genetiske udskiftning musemodeller ved hjælp af traditionelle embryonale stamcelle-baseret homologe rekombination-medieret målretning teknologi.
