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Visualização de variantes genéticas, metas de curtas e mutações pontuais no contexto morfológico do tecido com um ensaio de RNA In Situ da hibridação
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August 14, 2018
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Genetic VariantsShort TargetsPoint MutationsRNA In Situ HybridizationCell Type ExpressionSplice VariantsNeurocienciasOncologyExon JunctionsSensitivitySpecificitySingle CellsFormalin FixationParaffin EmbeddingMicrotome SectioningTarget RetrievalWash BufferHematoxylin CounterstainProbe HybridizationAmplification Reagents
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Anderson, C. M., Laeremans, A., Wang, X. M., Wu, X., Zhang, B., Doolittle, E., Kim, J., Li, N., Pimentel, H. X. Y., Park, E., Ma, X. Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay. J. Vis. Exp. (138), e58097, doi:10.3791/58097 (2018).
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