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Vitro Köpek nötrofil hücre dışı tuzak oluşumu: Floresans mikroskobu dinamik ve nicel analiz
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JoVE Revista Inmunología y contagio
In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy
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Vitro Köpek nötrofil hücre dışı tuzak oluşumu: Floresans mikroskobu dinamik ve nicel analiz

8,509 Views

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09:45 min

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August 24, 2018

DOI:

10.3791/58083-v

DOI:
10.3791/58083-v

•

09:45 min
August 24, 2018

•

4 Views
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1Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine,University of California, Davis, 2Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine,University of California, Davis

Summary

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Biz tam kan gelen köpek nötrofil yalıtmak ve NET canlı nötrofil floresans mikroskobu kullanılarak oluşumunda görselleştirmek için yöntemleri açıklanmaktadır. Ayrıca açıklanan NET oluşumu ve citrullinated histon H3 ölçmek için iletişim kurallarıdır ayirt mikroskobu kullanarak (citH3) ifade.

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Li, R. H., Tablin, F. In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy. J. Vis. Exp. (138), e58083, doi:10.3791/58083 (2018).

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