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体外犬中性粒细胞胞外陷阱的形成: 荧光显微镜动态定量分析
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy
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体外犬中性粒细胞胞外陷阱的形成: 荧光显微镜动态定量分析

8,509 Views

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09:45 min

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August 24, 2018

DOI:

10.3791/58083-v

DOI:
10.3791/58083-v

•

09:45 min
August 24, 2018

•

4 Views
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1Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine,University of California, Davis, 2Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine,University of California, Davis

Summary

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我们描述了从全血中分离犬中性粒细胞的方法, 用荧光显微镜观察活中性粒细胞的网状形成。还描述了使用免疫荧光显微镜定量分析网络形成和瓜组蛋白 H3 (citH3) 表达的协议。

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Li, R. H., Tablin, F. In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy. J. Vis. Exp. (138), e58083, doi:10.3791/58083 (2018).

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