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Quantitative Zellbiologie der Neurodegeneration in Drosophila durch unvoreingenommene Analyse der Gewebekulturen Tagged Proteine mit ImageJ
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Quantitative Zellbiologie der Neurodegeneration in Drosophila durch unvoreingenommene Analyse der Gewebekulturen Tagged Proteine mit ImageJ
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08:44 min
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August 03, 2018
DOI:
1Department of Molecular and Cellular Pharmacology,University of Miami Miller School of Medicine, 2School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong,Yantai University
Summary
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Wir haben einen einfachen und anpassungsfähigen Workflow zur Fluoreszenz-Imaging-basierte zellbiologischen Studien Proteinaggregation und selbstverdauende Fluss in das zentrale Nervensystem der Drosophila -Modelle von quantitativen Daten entlocken entwickelt. Neurodegeneration.
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Quantitative Cell BiologyNeurodegenerationDrosophilaFluorescently Tagged ProteinsImageJImage Analysis WorkflowProteinaceous PunctaMembrane-bound CompartmentsNeurodegenerative DiseasesLamina DissectionRetina RemovalBrain DissectionImmunostainingAntibody IncubationMountingMicroscopyFluorescence Imaging
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Cite this Article
Brazill, J. M., Zhu, Y., Li, C., Zhai, R. G. Quantitative Cell Biology of Neurodegeneration in Drosophila Through Unbiased Analysis of Fluorescently Tagged Proteins Using ImageJ. J. Vis. Exp. (138), e58041, doi:10.3791/58041 (2018).
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