This content is Free Access.
CRISPR Guide RNA Cloning for Mammalian Systems
66,749 Views
•
06:48 min
•
October 02, 2018
DOI:
1Wyss Institute for Biologically Inspired Engineering,Harvard University, 2Department of Genetics,Harvard Medical School, 3Department of Pathology,Massachusetts General Hospital, 4Institute for Medical Engineering & Science,Massachusetts Institute of Technology, 5Synthetic Biology Center,Massachusetts Institute of Technology, 6Department of Biological Engineering,Massachusetts Institute of Technology, 7Broad Institute
Summary
Automatically generated
Here, a simple, efficient, and cost-effective method of sgRNA cloning is outlined.
Read Article
Cite this Article
Nageshwaran, S., Chavez, A., Cher Yeo, N., Guo, X., Lance-Byrne, A., Tung, A., Collins, J. J., Church, G. M. CRISPR Guide RNA Cloning for Mammalian Systems. J. Vis. Exp. (140), e57998, doi:10.3791/57998 (2018).
Copy