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JoVE Journal Developmental Biology
Induction and Validation of Cellular Senescence in Primary Human Cells
 

Induction and Validation of Cellular Senescence in Primary Human Cells

Article DOI: 10.3791/57782-v • 08:18 min • June 20th, 2018
June 20th, 2018 •
1, 1, 1
1European Research Institute for the Biology of Aging, University of Groningen, University Medical Center Groningen

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Resumen
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Here, we discuss a series of protocols for induction and validation of cellular senescence in cultured cells. We focus on different senescence-inducing stimuli and describe the quantification of common senescence-associated markers. We provide technical details using fibroblasts as a model, but the protocols can be adapted to various cellular models.

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Keywords: Cellular Senescence Primary Human Cells Induction Validation Beta-galactosidase Staining Doxorubicin Fibroblasts Aging Cancer Cell Culture Microscopy
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