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Inmunología y contagio

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成像 Ca^{2 +}在志贺菌感染上皮细胞期间的响应

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Inmunología y contagio

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JoVE Revista Inmunología y contagio

Imaging Ca²⁺ Responses During Shigella Infection of Epithelial Cells

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成像 Ca^{2 +}在志贺菌感染上皮细胞期间的响应

5,360 Views

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08:56 min

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May 24, 2018

DOI:

10.3791/57728-v

DOI:

10.3791/57728-v

•

08:56 min

May 24, 2018

•

3 Views

Yasmine Smail^1,2,3,4, Chunhui Sun^1,2,3,4, Laurent Combettes⁵, Guy Tran Van Nhieu^1,2,3,4

¹Equipe Communication Intercellulaire et Infections Microbiennes, Centre de Recherche Interdisciplinaire en Biologie (CIRB),Collège de France, ²Institut National de la Santé et de la Recherche Médicale (Inserm), U1050, ³Centre Nationale de la Recherche Scientifique (CNRS), UMR7241, ⁴MEMOLIFE Laboratory of Excellence and Paris Sciences et Lettres, ⁵Inserm, UMRS1174,Université Paris Sud

Summary

在这里, 我们提出的协议, 可视化钙 (Ca^{2 +}) 的反应引起的 HeLa 细胞感染, 由志贺菌。通过对 ca^{2 +}荧光探针的细菌感染和成像参数进行优化, 提出了由细菌引起的非典型全局和局部 Ca^{2 +}信号在大范围的感染动力学中的特征。

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