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En Neurosphere analyse til at vurdere endogene neurale stamceller aktivering i en musemodel af Minimal rygmarvsskade
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JoVE Revista Neurociencias
A Neurosphere Assay to Evaluate Endogenous Neural Stem Cell Activation in a Mouse Model of Minimal Spinal Cord Injury

En Neurosphere analyse til at vurdere endogene neurale stamceller aktivering i en musemodel af Minimal rygmarvsskade

DOI:
10.3791/57727-v

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09:08 min

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September 13, 2018

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, ,
1Institute of Medical Science,University of Toronto, 2Department of Surgery,University of Toronto, 3Institute of Biomaterials and Biomedical Engineering,University of Toronto

Capítulos

  • 00:04Título
  • 00:45Minimal Spinal Cord Injury (SCI) Surgery
  • 04:01Spinal Cord Isolation and Neurosphere Culture
  • 07:18Results: Representative Neurosphere Analyses Following Minimal SCI
  • 08:24Conclusion

Summary

Traducción Automática

Traducción Automática

Her, viser vi udførelsen af en minimal rygmarv skade model i et voksen mus, der skåner den centrale kanal niche bolig endogene neurale stamceller (NSCs). Vi viser, hvordan neurosphere analysen kan bruges til at kvantificere aktivering og migration af endelige og primitive NSCs efter skade.

Tags

Neural Stem CellsSpinal Cord InjuryNeurosphere AssayEndogenous Neural Precursor CellsRegenerative MedicineMouse ModelSurgical Technique

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