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Calorespirometry: A Powerful, Noninvasive Approach to Investigate Cellular Energy Metabolism
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JoVE Revista Bioingeniería
Calorespirometry: A Powerful, Noninvasive Approach to Investigate Cellular Energy Metabolism

Calorespirometry: A Powerful, Noninvasive Approach to Investigate Cellular Energy Metabolism

DOI:
10.3791/57724-v

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09:08 min

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May 31, 2018

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, ,
1Department of Biological Sciences,University of Louisville, 2Department of Chemistry,Ball State University

Capítulos

  • 00:04Título
  • 00:51Respirometer and Colorimeter Preparation
  • 02:25Human Hepatocellular Carcinoma (HepG2) Cell Preparation
  • 04:26Calorimetry and Respirometry
  • 06:36Results: Representative O2 Concentration and Flux Analyses
  • 08:23Conclusion

Summary

Traducción Automática

Traducción Automática

This protocol describes calorespirometry, the direct and simultaneous measurement of both heat dissipation and respiration, which provides a noninvasive approach to assess energy metabolism. This technique is used to assess the contribution of both aerobic and anaerobic pathways to energy utilization by monitoring the total cellular energy flow.

Tags

Keywords: CalorespirometryCellular Energy MetabolismMitochondrial ToxicityPharmaceutical DevelopmentCryobiologyRespirometerCalorimetryOxygen ConsumptionHeat ProductionHepG2 Cells

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