Journal
/
Neurociencias
/
Ex Vivo Calcium Imaging for Visualizing Brain Responses to Endocrine Signaling in Drosophila
JoVE Revista
Neurociencias
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Neurociencias
Ex Vivo Calcium Imaging for Visualizing Brain Responses to Endocrine Signaling in Drosophila

Ex Vivo Calcium Imaging for Visualizing Brain Responses to Endocrine Signaling in Drosophila

DOI:
10.3791/57701-v

•

06:49 min

•

June 02, 2018

•

,
1Division of Biological Science, Graduate School of Science,Nagoya University, 2Department of Molecular Genetics, Institute of Life Science,Kurume University

Capítulos

  • 00:04Título
  • 00:31Preparation of Larval Brain Explants
  • 02:50Acquisition of Ca2+ Fluorescence Images
  • 04:00Data Analysis
  • 05:03Results: Calcium Imaging of the Larval Brain Explant
  • 05:53Conclusion

Summary

Traducción Automática

Traducción Automática

This paper describes a protocol for ex vivo calcium imaging of the Drosophila brain. In this method, natural or synthetic compounds can be applied to the buffer to test their ability to activate particular neurons in the brain.

Tags

Ex Vivo Calcium ImagingDrosophilaEndocrine SignalingBrain ResponsesNeurocienciasImaging ChamberGCaMPFluorescence Imaging

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Calcium Imaging of Odor-evoked Responses in the Drosophila Antennal Lobe

Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices

Ex Vivo Optogenetic Dissection of Fear Circuits in Brain Slices

In Vivo Functional Brain Imaging Approach Based on Bioluminescent Calcium Indicator GFP-aequorin

Ex Vivo Imaging of Cell-specific Calcium Signaling at the Tripartite Synapse of the Mouse Diaphragm

Preparing Adult Drosophila melanogaster for Whole Brain Imaging during Behavior and Stimuli Responses

In vivo Calcium Imaging of Mouse Geniculate Ganglion Neuron Responses to Taste Stimuli

In vivo Calcium Imaging in Mouse Inferior Olive

Brain Pericyte Calcium and Hemodynamic Imaging in Transgenic Mice In Vivo

Author Spotlight: Unveiling Neural Coding and Mechanisms of Visual Processing in the Superior Colliculus

Read Article