Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and  GTPase-linked Immunosorbent Assay

Javad Alizadeh; Shahla Shojaei; Simone da Silva Rosa; Adel Rezaei Moghadam; Amir A. Zeki; Mohammad Hashemi; Marek J. Los; Joseph W. Gordon; Saeid Ghavami

doi:10.3791/57646

/

Erkennung von kleine GTPase Prenylation und GTP binden mit Membran Fraktionierung und GTPase verbundene Immunosorbentprobe Assay

JoVE Revista
Bioquímica
Author Produced

Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.

JoVE Revista Bioquímica

Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay

Please note that all translations are automatically generated. Click here for the English version.

Erkennung von kleine GTPase Prenylation und GTP binden mit Membran Fraktionierung und GTPase verbundene Immunosorbentprobe Assay

9,644 Views

•

13:51 min

•

November 11, 2018

DOI:

10.3791/57646-v

DOI:

10.3791/57646-v

•

13:51 min

November 11, 2018

•

8 Views

Javad Alizadeh^1,2, Shahla Shojaei^1,2,3, Simone da Silva Rosa¹, Adel Rezaei Moghadam^1,2, Amir A. Zeki⁴, Mohammad Hashemi⁵, Marek J. Los^6,7,8, Joseph W. Gordon^1,9, Saeid Ghavami^1,2,10

¹Department of Human Anatomy and Cell Science, Rady Faculty of Health Sciences, Max Rady College of Medicine,University of Manitoba, ²Biology of Breathing Theme, Children Hospital Research Institute of Manitoba,University of Manitoba, ³Department of Biochemistry, School of Pharmacy and Pharmaceutical Sciences,Isfahan University of Medical Sciences, ⁴Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine,Center for Comparative Respiratory Biology and Medicine, ⁵Department of Clinical Biochemistry, School of Medicine,Zahedan University of Medical Sciences, ⁶Department of Molecular Biology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec,Medical University of Silesia, ⁷Centre de biophysique moléculaire – UPR 4301,Centre national de la recherche scientifique (CNRS) CS80054, ⁸LinkoCare Life Sciences AB, ⁹College of Nursing and Children’s Hospital Research Institute of Manitoba, Rady Faculty of Health Sciences,University of Manitoba, ¹⁰Health Policy Research Center, Institute of Health,Shiraz University of Medical Sciences

Summary

Hier beschreiben wir ein Protokoll, um die Prenylation und Guanosin-5'-Triphosphat (GTP) untersuchen-Laden von Rho GTPase. Dieses Protokoll besteht aus zwei detaillierte Methoden, nämlich Membran Fraktionierung und ein GTPase verbundene Immunosorbentprobe assay. Das Protokoll kann verwendet werden, zur Messung der Prenylation und GTP Laden von verschiedenen anderen kleinen GTPasen.

Read Article

Erkennung von kleine GTPase Prenylation und GTP binden mit Membran Fraktionierung und GTPase verbundene Immunosorbentprobe Assay

Summary

Automatically generated

Explore More Videos