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提耳伸肌压钳条件下哺乳动物神经肌肉传输的检查准备
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Levator Auris Longus Preparation for Examination of Mammalian Neuromuscular Transmission Under Voltage Clamp Conditions
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提耳伸肌压钳条件下哺乳动物神经肌肉传输的检查准备

9,137 Views

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10:45 min

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May 05, 2018

DOI:

10.3791/57482-v

DOI:
10.3791/57482-v

•

10:45 min
May 05, 2018

•

14 Views
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1Department of Biological Sciences,Wright State University

Summary

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本文所描述的协议使用鼠标提耳肌 (拉尔) 肌肉记录自发性和神经诱发突触后电位 (电流钳) 和电流 (电压钳) 在神经肌肉连接。使用该技术可以为正常和疾病条件下突触传递机制提供关键的见解。

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Levator Auris LongusNeuromuscular TransmissionVoltage ClampSynaptic CurrentsEnd Plate CurrentQuantal ContentProbability Of ReleaseNeurotransmissionMuscle ExcitabilitySynaptic FunctionNerve-muscle PreparationMammalianDissectionElectrophysiology

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Burke, S. R. A., Reed, E. J., Romer, S. H., Voss, A. A. Levator Auris Longus Preparation for Examination of Mammalian Neuromuscular Transmission Under Voltage Clamp Conditions. J. Vis. Exp. (135), e57482, doi:10.3791/57482 (2018).

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