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Uso de anticorpos Antifosfo-girdin Visualizar células intestinais topete no Mouse flutuante Jejunum Cryosections
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Biología
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JoVE Revista Biología
Use of Anti-phospho-girdin Antibodies to Visualize Intestinal Tuft Cells in Free-Floating Mouse Jejunum Cryosections

Uso de anticorpos Antifosfo-girdin Visualizar células intestinais topete no Mouse flutuante Jejunum Cryosections

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06:26 min

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March 21, 2018

DOI:

10.3791/57475-v

DOI:
10.3791/57475-v

•

06:26 min
March 21, 2018

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2 Views
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1Division of Perinatology, Institute for Developmental Research,Aichi Human Service Center, 2Surgery Department,Anjo Kosei Hospital, 3Department of Pathology,Nagoya University Graduate School of Medicine

Summary

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Kuga et al descobriram que anticorpos específicos de fosforilação-estatuto contra a ligação actina proteína girdin fosforilado em tirosina 1798 (pY1798) podem ser usados para rotular o topete células (TCs). Este protocolo permite visualização robusta de SCT usando coloração imunofluorescente de jejuno flutuantes cryosections com anticorpos pY1798.

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Cite this Article

Mizutani, Y., Kuga, D., Iida, M., Ushida, K., Takagi, T., Tokita, Y., Takahashi, M., Asai, M. Use of Anti-phospho-girdin Antibodies to Visualize Intestinal Tuft Cells in Free-Floating Mouse Jejunum Cryosections. J. Vis. Exp. (133), e57475, doi:10.3791/57475 (2018).

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