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腺相关病毒介导的脊髓基因神经元的转基因表达
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JoVE Revista Neurociencias
Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord
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腺相关病毒介导的脊髓基因神经元的转基因表达

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08:41 min

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May 12, 2018

DOI:

10.3791/57382-v

DOI:
10.3791/57382-v

•

08:41 min
May 12, 2018

•

4 Views
, , ,
1Institute of Pharmacology and Toxicology,University of Zurich, 2Institute of Pharmaceutical Sciences,Swiss Federal Institute (ETH) Zurich

Summary

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椎管内注射 recombinase 依赖重组腺相关病毒 (rAAV) 可以用来操纵任何基因标记的细胞类型的脊髓。在这里, 我们描述如何传感器神经元的背角的腰椎脊髓。这种技术可以对纵的神经元亚型进行功能性审问。

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Cite this Article

Haenraets, K., Albisetti, G. W., Foster, E., Wildner, H. Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord. J. Vis. Exp. (135), e57382, doi:10.3791/57382 (2018).

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