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Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes
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Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes
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February 02, 2018
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Staining DNA molecules for fluorescence microscopy allows a scientist to view them during an experiment. In the method presented here, DNA molecules are pre-stained with fluorescent dyes and digested with methylation and non-methylation sensitive restriction enzymes.
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Maschmann, A., Masters, C., Davison, M., Lallman, J., Thompson, D., Kounovsky-Shafer, K. L. Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes. J. Vis. Exp. (132), e57141, doi:10.3791/57141 (2018).
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