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人精切肺切片中物质的细胞毒性及免疫调节作用的评价
JoVE Revista
Inmunología y contagio
This content is Free Access.
JoVE Revista Inmunología y contagio
Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices
DOI:

12:04 min

May 09, 2018

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Capítulos

  • 00:05Título
  • 01:26General Preparation of Human PCLS and Subsequent Exposure to Chemicals
  • 04:28Measurement of Chemically-induced Cytotoxicity in Human PCLS by WST-1 Assay
  • 06:01LDH Assay
  • 07:29Microscopic Viability Assay with Confocal Laser Scanning Microscope (cLSM)
  • 09:49Results: Representative Images of the Three-dimensional Microscopic Staining and Image Rendering of Human PCLS
  • 10:59Conclusion

Summary

Traducción Automática

鉴于3Rs 原则, 呼吸道模型作为动物研究的替代品正在演变。特别是对于呼吸物质的风险评估, 缺乏适当的化验方法。在这里, 我们描述了使用人的精密切割肺切片评估机载物质。

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