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CRISPR/Cas9-mediated 目标集成在体内使用基于同源介导的端接连接策略
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08:22 min
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March 12, 2018
DOI:
1Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences, 2College of Life Sciences,University of Chinese Academy of Sciences, 3School of Life Science and Technology,Shanghai Tech University, 4Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences
Summary
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聚簇定期 interspaced 短回文重复/CRISPR 相关蛋白 9 (CRISPR/Cas9) 系统为基因工程提供了一个有前途的工具, 并开辟了转基因目标整合的可能性。我们描述了一种基于同源介导的端接 (HMEJ) 策略, 用于有效的 DNA 靶向集成体内和使用 CRISPR/Cas9 的靶向基因治疗。
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Cite this Article
Yao, X., Wang, X., Liu, J., Shi, L., Huang, P., Yang, H. CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy. J. Vis. Exp. (133), e56844, doi:10.3791/56844 (2018).
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