Journal
/
/
Monospecific 和混合 DFS70 模式在 HEp-2 ELITE/DFS70 击倒底物筛选中的同时鉴别
JoVE Revista
Inmunología y contagio
This content is Free Access.
JoVE Revista Inmunología y contagio
Simultaneous Distinction of Monospecific and Mixed DFS70 Patterns During ANA Screening with a Novel HEp-2 ELITE/DFS70 Knockout Substrate
DOI:

10:05 min

January 17, 2018

,

Capítulos

  • 00:05Título
  • 01:01Sample and Substrate Preparation
  • 02:04Addition of Controls and Samples
  • 03:37Addition of Fluorescent Conjugate and Analysis of Results
  • 06:32Results: Classic Disease Associated ANA and DFS70 Patterns on Conventional Hep-2 and Novel Hep-2 DFS70 Knockout Substrates
  • 08:20Conclusion

Summary

Traducción Automática

DFS70 自身抗体模拟常见的疾病相关的核抗体模式, 使准确的解释在使用传统的 HEp-2 基板的挑战。该协议描述了新的工程 HEp-2 基底优于传统的 HEp-2 在 ana 筛选和区分 DFS70 模式与高信心 monospecific 和混合 ana 阳性案件。

Videos relacionados

Read Article