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粘附细胞培养的时间推移荧光显微镜对蛋白质降解率的单细胞定量研究
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February 04, 2018
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该协议描述了一种方法, 以确定蛋白质半生命的单一生活粘附细胞, 使用脉冲标记和荧光时间推移成像的捕捉标签融合蛋白。
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Protein DegradationSingle-cellTime-lapse Fluorescence MicroscopyAdherent Cell CultureSNAP-tagProtein Half-lifeFluorescence ImagingProtein TurnoverHeterogeneityEndogenous Protein TaggingOverexpressionDNA Repair EnzymeBenzylguanineFluorescent DyeExponential DecayEmbryonic Stem CellsMonolayerCell-cell InteractionsN-cadherin
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Alber, A. B., Suter, D. M. Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture. J. Vis. Exp. (132), e56604, doi:10.3791/56604 (2018).
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