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Levende billeder af GLUT4 Protein handel med musen primære hypothalamus neuroner ved hjælp af Deconvolution mikroskopi
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1The Ph.D. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology,Taipei Medical University, 2Integrated Laboratory, Center of Translational Medicine,Taipei Medical University, 3Core Facility,Taipei Medical University, 4The Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University, 5Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University, 6TMU research center for Neurotrauma and Neuroregeneration, College of Medical Science and Technology,Taipei Medical University
Capítulos
- 00:05Título
- 01:00Primary Neuron Culture and Brain Tissue Isolation
- 02:56Tissue Digestion, Plating, and Culture
- 04:32Live Image Recording
- 06:48Results: The Snapshots of GFP-GLUT4 in Hypothalamic Neurons
- 07:47Conclusion
Denne protokol beskriver en teknik til observation af real-time grøn fluorescens Protein (NGL) mærket glukose Transporter 4 (GLUT4) protein handel på insulin stimulation og karakterisering af CCR5 biologiske rolle i insulin – GLUT4 signalering pathway med Deconvolution mikroskopi.
