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Etablering av Larval zebrafiskar som ett djur-modell för att undersöka Trypanosoma cruzi motilitet I Vivo
JoVE Revista
Biología del desarrollo
Author Produced
This content is Free Access.
JoVE Revista Biología del desarrollo
Establishment of Larval Zebrafish as an Animal Model to Investigate Trypanosoma cruzi Motility In Vivo
DOI:

13:21 min

September 30, 2017

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Capítulos

  • 00:00Título
  • 00:13Introduction
  • 03:09Obtaining Embryos for Injection
  • 04:13Preparing Injection Material
  • 05:38Obtaining Labeled T. cruzi Parasites for Injection
  • 07:04Injecting Zebrafish Larvae
  • 08:10LSFM Mounting and Imaging of Injected Larvae
  • 08:57LSFM Imaging of Injected Larvae
  • 10:44Representative Results
  • 11:40Conclusion

Summary

Traducción Automática

I detta protokoll, fluorescently märkt T. cruzi skulle injiceras i genomskinliga zebrafiskar larver och parasit motilitet observerades i vivo med lätta blad fluorescensmikroskopi.

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