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用丙烯酰胺凝胶电泳法可视化近红外荧光标记 dna 聚合酶活性

JoVE Revista
Bioquímica

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JoVE Revista Bioquímica

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

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用丙烯酰胺凝胶电泳法可视化近红外荧光标记 dna 聚合酶活性

13,919 Views

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07:38 min

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October 06, 2017

DOI:

10.3791/56228-v

DOI:

10.3791/56228-v

•

07:38 min

October 06, 2017

•

1 Views

Eliza L. Lewis¹, Aaron M. Leconte¹

¹Department of Chemistry,W.M. Keck Science Department of Claremont Mckenna, Pitzer, and Scripps College

Summary

本议定书描述了 dna 聚合酶合成的修饰 dna 通过观察的变化近红外荧光标记 dna 使用凝胶电泳和凝胶显像。丙烯酰胺凝胶用于高分辨率成像分离的短核酸, 这是根据大小不同的速率迁移。

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