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密なトランスポゾン挿入ライブラリを使用して細菌活用腸内細菌系統など、大腸菌や赤痢菌の作成
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri
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密なトランスポゾン挿入ライブラリを使用して細菌活用腸内細菌系統など、大腸菌や赤痢菌の作成

15,293 Views

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11:36 min

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September 23, 2017

DOI:

10.3791/56216-v

DOI:
10.3791/56216-v

•

11:36 min
September 23, 2017

•

3 Views
1Institute of Natural and Mathematical Sciences,Massey University

Tags

Transposon Insertion LibraryBacterial ConjugationEscherichia ColiShigella FlexneriTn10 TransposonDonor StrainRecipient StrainNitrocellulose FilterLB Agar PlateCell PelletResuspensionAseptic Technique

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Freed, N. E. Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri. J. Vis. Exp. (127), e56216, doi:10.3791/56216 (2017).

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