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एक ई. कोलाईआधारित कोशिका-मुक्त अभिव्यक्ति प्रणाली में संक्रामक Bacteriophages का संश्लेषण
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Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System
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एक ई. कोलाईआधारित कोशिका-मुक्त अभिव्यक्ति प्रणाली में संक्रामक Bacteriophages का संश्लेषण

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11:33 min

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August 17, 2017

DOI:

10.3791/56144-v

DOI:
10.3791/56144-v

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11:33 min
August 17, 2017

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2 Views
, , , ,
1School of Physics and Astronomy,University of Minnesota, 2Department of Diagnostic and Biological Sciences and Institute for Molecular Virology,University of Minnesota

Tags

Bacteriophage SynthesisE. Coli Cell-free Expression SystemPhage PlaquesPhage InfectionDNaseSucrose GradientUltracentrifugationPhage Band Extraction

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Rustad, M., Eastlund, A., Marshall, R., Jardine, P., Noireaux, V. Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System. J. Vis. Exp. (126), e56144, doi:10.3791/56144 (2017).

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