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Real-time In Vivo optagelse af Arabidopsis calciumsignaler under insekt fodring ved hjælp af en fluorescerende Biosensor
1Department of Metabolic Biology,John Innes Centre, Norwich Research Park, 2Department of Botany,University of Wisconsin, Madison, 3Department of Biochemistry and Molecular Biology,Saitama University, 4Precursory Research for Embryonic Science and Technology (PRESTO),Japan Science and Technology Agency (JST), 5Department of Cell and Developmental Biology,John Innes Centre, Norwich Research Park
Capítulos
- 00:05Título
- 00:44Arabidopsis Leaf Detachment
- 01:38Fluorescence Microscopy
- 03:06Data Collection
- 05:39Time-course Video Creation
- 06:39Results: Analysis of GCaMP3 Fluorescence to Detect Aphid-elicited Calcium Signals at the Feeding Site in Detached Arabidopsis Leaves
- 07:30Conclusion
Denne protokol beskriver en simpel metode til at analysere calciumsignaler i planter genereret ved at fodre hemipteran insekter såsom bladlus. Arabidopsis thaliana omdannet med normal god landbrugspraksis calcium biosensor GCaMP3 giver mulighed for real-time i vivo billeddannelse af calcium dynamics med en høj tidsmæssige og rumlige opløsning.
