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Journal / Developmental Biology / The C. elegans Excretory Canal as a Model for…
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Developmental Biology

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JoVE Journal Developmental Biology
The C. elegans Excretory Canal as a Model for Intracellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis in a Single Cell
 

The C. elegans Excretory Canal as a Model for Intracellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis in a Single Cell: labeling by GFP-fusions, RNAi Interaction Screen and Imaging

Article DOI: 10.3791/56101-v • 10:30 min • October 3rd, 2017
October 3rd, 2017 •
1,2, 1, 1, 1,3, 1, 1
1Mucosal Immunology and Biology Research Center, Developmental Biology and Genetics Core, Massachusetts General Hospital for Children, Harvard Medical School, 2College of Life Sciences, Jilin University, 3Faculty of Health Sciences, University of Macau

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The C. elegans excretory canal is a unique single-cell model for the visual in vivo analysis of de novo polarized membrane biogenesis. This protocol describes a combination of standard genetic/RNAi and imaging approaches, adaptable for the identification and characterization of molecules directing unicellular tubulogenesis, and apical membrane and lumen biogenesis.

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C. Elegans Excretory Canal Intracellular Lumen Morphogenesis In Vivo Polarized Membrane Biogenesis GFP-fusions RNAi Interaction Screen Imaging Tubulogenesis Polarity Apical Membrane Expansion Model System Osmotic Stress Imaging Manipulation Labeling Procedure Fluorescently Tagged Antibodies Live Imaging Analysis
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