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Using In Vivo and Tissue and Cell Explant Approaches to Study the Morphogenesis and Pathogenesis of the Embryonic and Perinatal Aorta
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Using In Vivo and Tissue and Cell Explant Approaches to Study the Morphogenesis and Pathogenesis of the Embryonic and Perinatal Aorta
Capítulos
- 00:05Título
- 01:07In Vivo Embryonic Fate Mapping and Clonal Analysis
- 04:14Explant Embryonic Aorta Culture
- 05:47Smooth Muscle Cell (SMC) Perinatal Aorta Isolation
- 07:16Subcutaneous Osmotic Mini-Pump Placement
- 08:23Results: Representative Embryonic and Neonatal SMC Analyses
- 09:47Conclusion
Protocols for studying the embryonic and perinatal murine aorta using in vivo clonal analysis and fate mapping, aortic explants, and isolated smooth muscle cells are detailed here. These diverse approaches facilitate the investigation of the morphogenesis of the embryonic and perinatal aorta in normal development and the pathogenesis in disease.
