Journal
/
Bioquímica
/
mRNA从植物原生质体捕获
JoVE Revista
Bioquímica
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Bioquímica
mRNA Interactome Capture from Plant Protoplasts
Please note that all translations are automatically generated. Click here for the English version.

mRNA从植物原生质体捕获

8,933 Views

•

12:29 min

•

July 28, 2017

DOI:

10.3791/56011-v

DOI:
10.3791/56011-v

•

12:29 min
July 28, 2017

•

4 Views
, , ,
1Department of Biology,KU Leuven

Summary

Automatically generated

在这里，我们提出了应用于拟南芥叶肉叶肉原生质体的相互作用捕获方案。该方法严格依赖于体内紫外线交联，并允许从生理环境中分离和鉴定植物mRNA结合蛋白。

Explore More Videos

MRNA InteractomeMRNA-bound ProteomePlant ProtoplastsPost-transcriptional Gene RegulationUV CrosslinkingArabidopsis ThalianaLeaf Mesophyll ProtoplastsMMG SolutionCentrifugation

Read Article

Cite this Article

Zhang, Z., Boonen, K., Li, M., Geuten, K. mRNA Interactome Capture from Plant Protoplasts. J. Vis. Exp. (125), e56011, doi:10.3791/56011 (2017).

Download .ris file

Copy

Share Video

.

Copy