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Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization

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Neurociencias

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JoVE Revista Neurociencias

Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization

Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization

7,953 Views

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09:30 min

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July 13, 2017

DOI:

10.3791/55924-v

DOI:

10.3791/55924-v

•

09:30 min

July 13, 2017

•

2 Views

Xiao Xu, Yinwei You², Long Zhang

¹Department of Entomology,China Agricultural University, ²Bio-tech Research Center,Shandong Academy of Agricultural Sciences

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Odorant Receptor Genes RNA In Situ Hybridization Locust Antennae Digoxigenin Labeled Probes Biotin Labeled Probes Freezing Microtome Cryostat Sections 4% PFA 0.2 M HCl 1X PBS 1% Triton X-100 Formamide Antisense Probe Sense Probe 0.1X SSC 1X TBS 1% Blocking Reagent Chromogenic Detection

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