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Live-cell mätning av luktämnet Receptor aktiveringen med en realtid cAMP-analys
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1Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of National Ministry of Education,Shanghai Jiao Tong University School of Medicine, 2Department of Molecular Genetics and Microbiology,Duke University Medical Center, 3Department of Neurobiology, Duke Institute for Brain Sciences,Duke University Medical Center, 4Institute of Health Science,Chinese Academy of Science/Shanghai Jiao Tong University School of Medicine
Capítulos
- 00:05Título
- 00:50Plating and Transfection of Hana3A Cells
- 03:03Transfection of Plasmids
- 05:24Stimulation and Measuring OR Activity Using the Real-time cAMP Assay
- 07:27Results: Kinetic Measurements of the Response of OR5AN1 to Muscone
- 08:23Conclusion
