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一种简单的分级提取方法，用于从单个样品中综合分析代谢物，脂质和蛋白质

JoVE Revista
Bioquímica

This content is Free Access.

JoVE Revista Bioquímica

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

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一种简单的分级提取方法，用于从单个样品中综合分析代谢物，脂质和蛋白质

DOI:

10.3791/55802-v

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11:17 min

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June 01, 2017

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Mohamed Salem², Michal Bernach, Krzysztof Bajdzienko, Patrick Giavalisco

¹Max Planck Institute of Molecular Plant Physiology, Golm, Germany, ²Department of Pharmacognosy, Faculty of Pharmacy,Cairo University

Capítulos

00:05Título
01:05Grinding and Tissue Disruption
02:30Aliquoting Tissue
03:18Extraction and Fractionation by Phase Separation
04:56Aliquoting Polar and Hydrophobic Fractions
06:24Concentrations and Storage of Fraction and Analysis of Lipids Using UPLC-MS
07:48Analysis of Polar and Semi-polar Metabolites Using UPLC-MS
08:51Results: Representative Chromatograms of Lipid, Semi-polar Metabolites and Proteins from Arabidopsis thaliana Leaf Extracts
10:40Conclusion

Summary

Traducción Automática

提出了使用一个样品从生物组织中综合提取脂质，代谢物和蛋白质的方案。

Tags

Keywords: Fractionated Extraction Metabolites Lipids Proteins Single Sample MTBE Extraction Multiomics Analysis Arabidopsis Sample Preparation

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