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Ex Vivo Imagem de linfócitos de T CD8 residente no Tumor de pulmão humano fatias utilizando microscopia Confocal
This content is Free Access.
1Institut Cochin, Inserm U1016-CNRS UMR8104,Université Paris Descartes, 2Department of Pathology, Paris Centre University Hospitals,Université Paris Descartes, 3INSERM U1138, Cancer and Immune Escape, Cordeliers Research Center,University Pierre and Marie Curie, 4Department of Thoracic Surgery, Paris Centre University Hospitals,University Paris Descartes
Capítulos
- 00:05Título
- 01:01Agarose Embedding and Vibratome Slicing of Human Lung Tumors
- 03:51Immunostaining of Resident CD8 T Cells and Tumor Cells
- 05:07Imaging and Analyzing Resident CD8 T Cell Migration Within Human Lung Tumor Slices
- 07:03Results: Distribution and Motility Patterns of Resident CD8 T Cells in Human Lung Tumor Tissues
- 07:53Conclusion
Este protocolo descreve um método de imagem residente tumor infiltrando T CD8 células rotulado com anticorpos fluorescente acoplados dentro de fatias de tumor de pulmão humano. Esta técnica permite análises em tempo real de migração de células T CD8 usando microscopia confocal.
