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Ex Vivo Avbildning av bosatt CD8 T-lymfocytter i menneskelig lunge svulst skiver bruker AC Confocal mikroskopi
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1Institut Cochin, Inserm U1016-CNRS UMR8104,Université Paris Descartes, 2Department of Pathology, Paris Centre University Hospitals,Université Paris Descartes, 3INSERM U1138, Cancer and Immune Escape, Cordeliers Research Center,University Pierre and Marie Curie, 4Department of Thoracic Surgery, Paris Centre University Hospitals,University Paris Descartes
Capítulos
- 00:05Título
- 01:01Agarose Embedding and Vibratome Slicing of Human Lung Tumors
- 03:51Immunostaining of Resident CD8 T Cells and Tumor Cells
- 05:07Imaging and Analyzing Resident CD8 T Cell Migration Within Human Lung Tumor Slices
- 07:03Results: Distribution and Motility Patterns of Resident CD8 T Cells in Human Lung Tumor Tissues
- 07:53Conclusion
Denne protokollen beskriver en metode for å bilde bosatt svulst-infiltrere CD8 T celler merket med fluorescently kombinert antistoffer i menneskelig lunge svulst skiver. Denne teknikken tillater sanntid analyser av CD8 T celle overføring ved hjelp av AC confocal mikroskopi.
