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En IL-8 Transient Transgenized Mouse Model for<em> In vivo</em> Langsigtet overvågning af inflammatoriske reaktioner
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1Department of Medicine, General Pathology Division, Cystic Fibrosis Translational Research Laboratory “D. Lissandrini”,University of Verona, 2Corporate Preclinical R&D,Chiesi Farmaceutici S.p.A., 3Department of Diagnostic and Public Health, Microbiology Division,University of Verona, 4Dipartimento di Scienze Medico-Veterinarie,University of Parma, 5Department of Biomedical Biotechnological and Translational Sciences,University of Parma, 6Department of Computer Science,University of Verona, 7Department of Neurological, Biomedical and Movement Sciences,University of Verona, 8Cystic Fibrosis Regional Center (CFC),AOUI Verona
Capítulos
- 00:05Título
- 01:07In Vivo Gene Delivery and Biomluminescent Imaging (BLI) and Photon Quantitification
- 04:24Pro-inflammatory Stimuli Mouse Challenge
- 06:51Results: Representative P. aeruginosa-induced Lung Inflammation Supernatent In Vivo Imaging in IL-8 Transiently Trangenic Mice
- 07:42Conclusion
Fremgangsmåden beskrevet her tillader visualisering af IL-8-promotorafhængig inflammationsaktivering i lungerne af mus gennem ikke-invasiv bioluminescensbilleddannelse (BLI). Det samme dyr kan udsættes for BLI flere gange i op til to måneder fra leveringstidspunktet for luciferase-reporterkonstruktionen.
