Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
جنبا إلى جنب تقارب تنقية البروتين المجمعات من خلايا حقيقية النواة
14,679 Views
•
11:30 min
•
January 26, 2017
DOI:
Summary
Automatically generated
We describe here a novel, robust, and efficient tandem affinity purification (TAP) method for the expression, isolation, and characterization of protein complexes from eukaryotic cells. This protocol could be utilized for the biochemical characterization of discrete complexes as well as the identification of novel interactors and post-translational modifications that regulate their function.
Read Article
Cite this Article
Ma, Z., Fung, V., D'Orso, I. Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells. J. Vis. Exp. (119), e55236, doi:10.3791/55236 (2017).
Copy