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Human Lung dendritische cellen: ruimtelijke verdeling en fenotypische Identification in Endobronchiale biopten middels immunohistochemie en flowcytometrie
JoVE Revista
Inmunología y contagio
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JoVE Revista Inmunología y contagio
Human Lung Dendritic Cells: Spatial Distribution and Phenotypic Identification in Endobronchial Biopsies Using Immunohistochemistry and Flow Cytometry

Human Lung dendritische cellen: ruimtelijke verdeling en fenotypische Identification in Endobronchiale biopten middels immunohistochemie en flowcytometrie

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11:02 min

January 20, 2017

DOI:

11:02 min
January 20, 2017

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Summary

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Lung-resident immune cells, including dendritic cells (DCs) in humans, are critical for defense against inhaled pathogens and allergens. However, due to the scarcity of human lung tissue, studies are limited. This work presents protocols to process human mucosal endobronchial biopsies for studying lung DCs using immunohistochemistry and flow cytometry.

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