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人肺树突状细胞：空间分布与表型鉴定支气管活检采用免疫组化和流式细胞仪
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Human Lung Dendritic Cells: Spatial Distribution and Phenotypic Identification in Endobronchial Biopsies Using Immunohistochemistry and Flow Cytometry
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人肺树突状细胞：空间分布与表型鉴定支气管活检采用免疫组化和流式细胞仪

9,104 Views

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11:02 min

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January 20, 2017

DOI:

10.3791/55222-v

DOI:
10.3791/55222-v

•

11:02 min
January 20, 2017

•

1 Views
, , , , , ,
1Immunology and Allergy Unit, Department of Medicine Solna,Karolinska Institutet, 2Division of Medicine, Department of Public Health and Clinical Medicine,Umeå University, 3Divison of Infectious Diseases, Department of Clinical Microbiology,Umeå University

Summary

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Lung-resident immune cells, including dendritic cells (DCs) in humans, are critical for defense against inhaled pathogens and allergens. However, due to the scarcity of human lung tissue, studies are limited. This work presents protocols to process human mucosal endobronchial biopsies for studying lung DCs using immunohistochemistry and flow cytometry.

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Cite this Article

Baharom, F., Rankin, G., Scholz, S., Pourazar, J., Ahlm, C., Blomberg, A., Smed-Sörensen, A. Human Lung Dendritic Cells: Spatial Distribution and Phenotypic Identification in Endobronchial Biopsies Using Immunohistochemistry and Flow Cytometry. J. Vis. Exp. (119), e55222, doi:10.3791/55222 (2017).

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