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תאים יחידים Gene Expression Profiling שימוש FACS ו qPCR עם סטנדרטים פנימיים
Capítulos
- 00:05Título
- 00:56Preparation of Standards from Purified DNA Amplicons and Treatment of MCF7 p53-Venus Cells
- 01:53Preparation of Lysis Buffer for Cell Sorting
- 04:15Cell Sorter Setup, Cell Harvesting, and Sorting
- 05:47Exonuclease Treatment and Sort Quality Control Using qPCR
- 07:02Gene Expression Measurement Using Microfluidic qPCR
- 09:03Results: Gene Expression Profiling in Single Cells Using FACS Analysis and qPCR
- 10:09Conclusion
