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Biología del desarrollo
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在成熟的肌纤维诱导分化实时成像
JoVE Revista
Biología del desarrollo
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JoVE Revista Biología del desarrollo
In Vitro Differentiation of Mature Myofibers for Live Imaging
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在成熟的肌纤维诱导分化实时成像

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08:12 min

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January 07, 2017

DOI:

10.3791/55141-v

DOI:
10.3791/55141-v

•

08:12 min
January 07, 2017

•

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1Instituto de Medicina Molecular, Faculdade de Medicina,Universidade de Lisboa, 2Myology Research Center, UM76-INSERM U974-CNRS FRE 361,Sorbonne University, UPMC University of Paris 6

Summary

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Muscle cells are among the most complex eukaryotic cells. We present a protocol for the in vitro differentiation of highly mature myofibers that allows for genetic manipulation and clear imaging during all developmental stages.

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Pimentel, M. R., Falcone, S., Cadot, B., Gomes, E. R. In Vitro Differentiation of Mature Myofibers for Live Imaging. J. Vis. Exp. (119), e55141, doi:10.3791/55141 (2017).

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