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应用先进的背皮窗室对转基因荧光小鼠肿瘤相关血管的活体显微镜观察

JoVE Revista
Medicina

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JoVE Revista Medicina

Intravital Microscopy of Tumor-associated Vasculature Using Advanced Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice

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应用先进的背皮窗室对转基因荧光小鼠肿瘤相关血管的活体显微镜观察

13,259 Views

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08:52 min

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January 19, 2018

DOI:

10.3791/55115-v

DOI:

10.3791/55115-v

•

08:52 min

January 19, 2018

•

10 Views

Ann L.B. Seynhaeve¹, Timo L.M. ten Hagen¹

¹Laboratory Experimental Surgical Oncology, Section Surgical Oncology, Department of Surgery,Erasmus MC

Summary

该协议描述了表达细胞特异荧光标记的转基因小鼠的活体成像。活体成像提供了一种非侵入性的方法, 用于高分辨率观察活体动物使用植入窗口, 通过它的微观可视化过程是可能的。这种方法对于研究纵向过程尤其有用。

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