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Génération de cellules humaines dérivées de monocytes dendritiques du sang total
JoVE Revista
Inmunología y contagio
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JoVE Revista Inmunología y contagio
Generation of Human Monocyte-derived Dendritic Cells from Whole Blood

Génération de cellules humaines dérivées de monocytes dendritiques du sang total

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07:35 min

December 24, 2016

DOI:

07:35 min
December 24, 2016

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Summary

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Here, we demonstrate how monocytes are isolated by magnetic bead separation from peripheral blood mononuclear cells after density gradient centrifugation of human anti-coagulated blood. Following incubation for 5 days, human monocytes are differentiated into immature dendritic cells and are ready for experimental procedures in a non-clinical setting.

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