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Biochemistry

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JoVE Journal Biochemistry
Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline
 

Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline

Article DOI: 10.3791/54861-v • 11:09 min • January 5th, 2017
January 5th, 2017 •
1, 2,3, 4
1Structural Biology Group, European Synchrotron Radiation Facility, 2European Molecular Biology Laboratory, 3School of Chemical and Physical Sciences, Keele University, 4Groupe de Microscopie Electronique et Méthodes, Institut de Biologie Structurale

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The determination of the solution structure of a protein by small angle X-ray scattering (SAXS) requires monodisperse samples. Here, we present two possibilities to ensure minimal delays between sample preparation and data acquisition: online size-exclusion chromatography (SEC) and online ion-exchange chromatography (IEC).

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Keywords: Online Size-exclusion Chromatography Ion-exchange Chromatography SAXS Beamline Structural Biology SAXS Molar Dispersed Samples SEC Gel Filtration Buffer HPLC System SAXS Flow-through Capillary Radiation Damage Protein Sample Aggregates HPLC Compatible Glass Vial UV Vis Data
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