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Cancer Research

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JoVE Journal Cancer Research
Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures
 

Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures

Article DOI: 10.3791/54738-v • 07:48 min • December 26th, 2016
December 26th, 2016 •
1,2, 1,2, 1,2, 1,2, 1,2, 3, 1, 1,2, 1,2
1The Breast Cancer Now Toby Robins Research Centre, Division of Breast Cancer, The Institute of Cancer Research, 2Division of Molecular Pathology, The Institute of Cancer Research, 3Institute of Cancer Sciences, University of Manchester

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Identifying novel drug targets that transition from pre-clinical testing to human trials is a scientific priority. To that end, here we describe a functional genomics approach for examining the impact of gene depletion on cancer cell line spheroids, which more appropriately model human cancers in vivo.

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Functional Genomics Screening Novel Drug Targets Cancer Cell Spheroid Cultures SiRNA Screen Protocol Gene Silencing Cancer Spheroids Two-dimensional Cell Culture Three-dimensional Culture Hypoxia Targeted SRNA Screening Biometric Dyes Apoptosis 96-well Plates SiRNA Library
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