Journal
/
Neurociencias
/
Recording Gamma Band Oscillations in Pedunculopontine Nucleus Neurons
JoVE Revista
Neurociencias
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Neurociencias
Recording Gamma Band Oscillations in Pedunculopontine Nucleus Neurons

Recording Gamma Band Oscillations in Pedunculopontine Nucleus Neurons

DOI:
10.3791/54685-v

•

09:04 min

•

September 14, 2016

•

, , , ,
1IFIBYNE-CONICET,University of Buenos Aires, 2Center for Translational Neuroscience,University of Arkansas for Medical Sciences

Capítulos

  • 00:05Título
  • 00:54Solutions Preparation
  • 02:07Slice Preparation
  • 03:12Whole-Cell Patch Clamp Recordings
  • 06:32Results: Gamma Band Membrane Oscillations in Whole-Cell Recorded PPN Neurons Using Current Ramps of Augmenting Amplitude
  • 07:44Conclusion

Summary

Traducción Automática

Traducción Automática

The pedunculopontine nucleus (PPN) is located in the brainstem and its neurons are maximally activated during waking and rapid eye movement (REM) sleep brain states. This work describes the experimental approach to record in vitro gamma band subthreshold membrane oscillation in PPN neurons.

Tags

Gamma Band OscillationsPedunculopontine Nucleus NeuronsIntrinsic Membrane PropertiesWhole-cell Patch Clamp RecordingACSF PreparationSucrose ACSFVibratome SlicingBrain Dissection

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Slice Preparation, Organotypic Tissue Culturing and Luciferase Recording of Clock Gene Activity in the Suprachiasmatic Nucleus

Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices

Recording Electrical Activity from Identified Neurons in the Intact Brain of Transgenic Fish

Intracellular Recording, Sensory Field Mapping, and Culturing Identified Neurons in the Leech, Hirudo medicinalis

Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons

Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor

Tuning in the Hippocampal Theta Band In Vitro: Methodologies for Recording from the Isolated Rodent Septohippocampal Circuit

Optogenetic Entrainment of Hippocampal Theta Oscillations in Behaving Mice

Read Article