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Karakterisering van Multi-subunit eiwitcomplexen of Human MxA het gebruik van niet-denaturerende Polyacrylamide Gel-elektroforese
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Karakterisering van Multi-subunit eiwitcomplexen of Human MxA het gebruik van niet-denaturerende Polyacrylamide Gel-elektroforese
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October 28, 2016
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This article describes a simple and rapid protocol to evaluate the oligomeric state of the dynamin-like GTPase MxA protein from lysates of human cells using a combination of non-denaturing PAGE with western blot analysis.
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Nigg, P. E., Pavlovic, J. Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis. J. Vis. Exp. (116), e54683, doi:10.3791/54683 (2016).
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