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Forberedelse og<em> In vivo</em> Anvendelse af en aktivitetsbaseret Probe til<em> N</em> -acylethanolamine Acid-amidase
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Capítulos
- 00:05Título
- 01:11Preparation of 2-pyridyl Undec-10-ynyl Carbonate and Undec-10-ynyl 2-oxopyridine 1-carboxylate
- 02:44Preparation of Undec-10-ynyl-N-[(3S)-2-oxoazetidin-3-yl]carbamate
- 04:17Click Chemistry with Azide-PEG3-Biotin
- 05:14Removal of Excess Click Chemistry Reagents
- 06:29Streptavidin Enrichment
- 07:57Click Chemistry with Azide-PEG3-Fluor 545 on Tissue Slices
- 08:59Results: Synthesis and Applications of NAAA Activity Based Probe, ARN14686
- 10:21Conclusion
Her beskriver vi fremstilling og anvendelse af et aktivitetsbaseret sonde (ARN14686, undec-10-ynyl- N - [(3 S) -2-oxoazetidin-3-yl] carbamat), der giver mulighed for påvisning og kvantificering af aktive form af det proinflammatoriske enzym N -acylethanolamine syre amidase (NAAA), både in vitro og ex vivo.
