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와 감염시 배수 림프절에 쥐 피부 수지상 세포의 마이그레이션을 연구하는 CFSE 기반 분석<em> 소 결핵균</em> Bacille 칼 메트 - GUERIN
JoVE Revista
Inmunología y contagio
This content is Free Access.
JoVE Revista Inmunología y contagio
A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Guérin
DOI:

07:14 min

October 09, 2016

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Capítulos

  • 00:05Título
  • 00:56Bacille Calmette-Guérin (BCG) and CFSE Injection
  • 02:08Popliteal Lymph Node (pLN) Harvest, Single Cell Suspension Preparation and Cell Surface Staining
  • 04:05Results: Migratory Skin Dendritic Cell Draining LN Relocation after BCG Footpad Infection
  • 06:04Conclusion

Summary

Traducción Automática

An assay in mice to track cell migration from skin to draining lymph node is described which enables the characterization of skin Dendritic cells mobilized to the lymph node after footpad infection with Bacille Calmette-Guérin.

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