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Capítulos
- 00:05Título
- 00:51Create Barriers from Aluminum Foil
- 02:08‘Window’ the Incubated Chicken Eggs
- 03:35Barrier to Prevent Leg Bud Initiation and Bead Operations at Stage 15
- 05:20Barrier to Prevent Leg Bud Induction and Initiation at Stage 10
- 06:34Results: Retinoic Acid from the Somites Is Essential for Limb Bud Outgrowth
- 07:36Conclusion
Protocols using impermeable barriers to block induction events between tissues of the main body axis and flank in the chick embryo required for limb formation are described. Beads soaked in candidate inductive signals are used to overcome the effect of barrier placement and analysis of gene expression confirms this.
